Maybe, maybe not.
The story of Eden, apple or not, they sinned. For example, (not saying what I currently believe or believe is the truth of Eden, but this is merely an example), lets say the trees were symbolic of books, scrolls, angels who would teach, etc.
Romans 14:1 Welcome the [man] having weaknesses in [his] faith, but not to make decisions on inward questionings. 2 One [man] has faith to eat everything, but the [man] who is weak eats vegetables. (Here we see in regards to spiritual food, the act of eating as symbolized by it. One for 'everything', and one for vegetables).
Romans 14:13 Therefore let us not be judging one another any longer, but rather make this YOUR decision, not to put before a brother a stumbling block or a cause for tripping. 14 I know and am persuaded in the Lord Jesus that nothing is defiled in itself; only where a man considers something to be defiled, to him it is defiled.
Romans 14:22Happy is the man that does not put himself on judgment by what he approves. 23 But if he has doubts, he is already condemned if he eats, because [he does] not [eat] out of faith. Indeed, everything that is not out of faith is sin.
Romans 3:19 - (Romans 3:19) Now we know that all the things the Law says it addresses to those under the Law, so that every mouth may be stopped and all the world may become liable to God for punishment. - (So what does the Law ACCOMPLISH here?)
(1 Corinthians 15:56) The sting producing death is sin, but the power for sin is the Law. (What produces death? What does it get its POWER from?)
(Galatians 2:16) knowing as we do that a man is declared righteous, not due to works of law, but only through faith toward Christ Jesus, even we have put our faith in Christ Jesus, that we may be declared righteous due to faith toward Christ, and not due to works of law, because due to works of law no flesh will be declared righteous. - (The law says just ONE sin equals what?
(Hebrews 10:26) For if we PRACTICE SIN willfully after having received the accurate KNOWLEDGE of the truth(Good and Evil?), there is no longer any sacrifice for sins left.
So, back to Garden of Eden and eating of the tree. Whether the fruit was real and made their minds realize good/bad, OR they took in knowledge they were not supposed to have, because by being like God, KNOWING good and evil, things started to become sin for them. Just the act of taking in that knowledge when the only thing they were told not to do, was the first sin, and started the curse of death. As seen in the above scriptures, the law exposes all sin, and impossible to not err even once for us.
Those on earth now who may want to reject knowing the truth, are not free either, Romans 2:12 - For instance, all those who sinned without law will also perish without law; but all those who sinned under law will be judged by law. 13 For the hearers of law are not the ones righteous before God, but the doers of law will be declared righteous. 14 For whenever people of the nations that do not have law do by nature the things of the law, these people, although not having law, are a law to themselves. 15 They are the very ones who demonstrate the matter of the law to be written in their hearts, while their conscience is bearing witness with them and, between their own thoughts, they are being accused or even excused. 16 This will be in the day when God through Christ Jesus judges the secret things of mankind, according to the good news I declare.