initially no, but those who work with or go to school with the LBGT community may have to have uncomfortable discussions about this. They may not feel as passive towards witnesses as they once did. Because it isn't a watchtower quote about god not approving of homosexuality, its a cartoon that addresses gay marriage in a specific way.
By putting this in a cartoon, they have chosen a very offensive medium (kids cartoon) for delivering their doctrine, also one that is easily spread. I don't care what HQ thinks about it or most R&F, I like to think that those who would normally say they know witnesses and they never hurt anybody start to see witnesses as more overtly harmful going forward.
I've been reading most of the articles posted in news and blogs (certainly, all links I can find on this forum, and others). Most stories are repetitive, but the Comments sections show a sometimes visceral reaction. You, fmf, have already described how some folks (gay, some nongay Xtians) are reacting. Some are okay with it, others are horrified. Yes, the message of this video is waking up some 'worldly' people to the insidious evil that is JW.